Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 65, Issue 20, Pages 5795-5809Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/eru313
Keywords
Abscisic acid (ABA); antioxidant defence; C2H2-type zinc finger protein; oxidative stress; rice; water stress
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Funding
- National Basic Research Program of China [2012CB114306]
- Fundamental Research Funds for the Central Universities [KYZ201157, KYTZ201402]
- National Natural Science Foundation of China [31070254, 31271631]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Research Fund for the Doctoral Program of Higher Education of China [20130097110025]
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C2H2-type zinc finger proteins (ZFPs) have been shown to play important roles in the responses of plants to oxidative and abiotic stresses, and different members of this family might have different roles during stresses. Here a novel abscisic acid (ABA)- and hydrogen peroxide (H2O2)-responsive C2H2-type ZFP gene, ZFP36, is identified in rice. The analyses of ZFP36-overexpressing and silenced transgenic rice plants showed that ZFP36 is involved in ABA-induced up-regulation of the expression and the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX). Overexpression of ZFP36 in rice plants was found to elevate the activities of antioxidant enzymes and to enhance the tolerance of rice plants to water stress and oxidative stress. In contrast, an RNA interference (RNAi) mutant of ZFP36 had lower activities of antioxidant enzymes and was more sensitive to water stress and oxidative stress. ABA-induced H2O2 production and ABA-activated mitogen-activated protein kinases (MAPKs) were shown to regulate the expression of ZFP36 in ABA signalling. On the other hand, ZFP36 also regulated the expression of NADPH oxidase genes, the production of H2O2, and the expression of OsMPK genes in ABA signalling. These results indicate that ZFP36 is required for ABA-induced antioxidant defence, for the tolerance of rice plants to water stress and oxidative stress, and for the regulation of the cross-talk between NADPH oxidase, H2O2, and MAPK in ABA signalling.
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