Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 65, Issue 12, Pages 3235-3248Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/eru171
Keywords
gamma-Aminobutyric acid; Ca2+-permeable channel; cell-cell communication; glutamate decarboxylase; Nicotiana tabacum; pollen tube
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Funding
- Major Research Plan from the Ministry of Science and Technology of China [2013CB945100]
- National Natural Science Foundation of China [31270361]
- OPTICHINA research fellowship in Rothamsted Research - (EU)
- OPTICHINA research fellowship in Rothamsted Research - (CAAS)
- China Scholarship Council Project for young outstanding talents
- Academic Innovation Team for Plant Development and Genetics in the South-Central University for Nationalities
- US National Science Foundation [IOS-0723421]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1146090] Funding Source: National Science Foundation
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gamma-Aminobutyric acid (GABA) is implicated in pollen tube growth, but the molecular and cellular mechanisms that it mediates are largely unknown. Here, it is shown that exogenous GABA modulates putative Ca2+-permeable channels on the plasma membranes of tobacco pollen grains and pollen tubes. Whole-cell voltage-clamp experiments and non-invasive micromeasurement technology (NMT) revealed that the influx of Ca2+ increases in pollen tubes in response to exogenous GABA. It is also demonstrated that glutamate decarboxylase (GAD), the rate-limiting enzyme of GABA biosynthesis, is involved in feedback controls of Ca2+-permeable channels to fluctuate intracellular GABA levels and thus modulate pollen tube growth. The findings suggest that GAD activity linked with Ca2+-permeable channels relays an extracellular GABA signal and integrates multiple signal pathways to modulate tobacco pollen tube growth. Thus, the data explain how GABA mediates the communication between the style and the growing pollen tubes.
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