Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 65, Issue 22, Pages 6711-6721Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/eru395
Keywords
Aristidoideae; herbarium; Madagascar; NGS; plastid genome; Poaceae; ribosomal DNA; Sartidia; single-copy genes; Stipagrostis
Categories
Funding
- CEBA [ANR-10-LABX-0025]
- METABAR [ANR-11-BSV7-0020]
- BBSRC
- NERC
- National Geographic Society Global Exploration Fund, Northern Europe grant [GEFNE10-11]
- Royal Society University [UF120119]
- SYNTHESYS programme [FR-TAF-2694]
- Agence Nationale de la Recherche (ANR) [ANR-11-BSV7-0020] Funding Source: Agence Nationale de la Recherche (ANR)
- Royal Society [UF120119] Funding Source: Royal Society
Ask authors/readers for more resources
Collections of specimens held by natural history museums are invaluable material for biodiversity inventory and evolutionary studies, with specimens accumulated over 300 years readily available for sampling. Unfortunately, most museum specimens yield low-quality DNA. Recent advances in sequencing technologies, so called next-generation sequencing, are revolutionizing phylogenetic investigations at a deep level. Here, the Illumina technology (HiSeq) was used on herbarium specimens of Sartidia (subfamily Aristidoideae, Poaceae), a small African-Malagasy grass lineage (six species) characteristic of wooded savannas, which is the C-3 sister group of Stipagrostis, an important C-4 genus from Africa and SW Asia. Complete chloroplast and nuclear ribosomal sequences were assembled for two Sartidia species, one of which (S. perrieri) is only known from a single specimen collected in Madagascar 100 years ago. Partial sequences of a few single-copy genes encoding phosphoenolpyruvate carboxylases (ppc) and malic enzymes (nadpme) were also assembled. Based on these data, the phylogenetic position of Malagasy Sartidia in the subfamily Aristidoideae was investigated and the biogeographical history of this genus was analysed with full species sampling. The evolutionary history of two genes for C-4 photosynthesis (ppc-aL1b and nadpme-IV) in the group was also investigated. The gene encoding the C-4 phosphoenolpyruvate caroxylase of Stipagrostis is absent from S. dewinteri suggesting that it is not essential in C-3 members of the group, which might have favoured its recruitment into a new metabolic pathway. Altogether, the inclusion of historical museum specimens in phylogenomic analyses of biodiversity opens new avenues for evolutionary studies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available