Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 64, Issue 16, Pages 4953-4966Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ert284
Keywords
2-DE; DGE; dormancy; GA4; Japanese apricot; proteomics; transcriptomics
Categories
Funding
- Jiangsu Province scientific research and innovation projects for postgraduates [CXZZ12_0283]
- Natural Science Foundation of Jiangsu Province [BK2011642]
- National Science Foundation of China [31101526]
- Priority Academic Programme Development of Jiangsu Higher Education Institutions (PAPD)
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Hormones are closely associated with dormancy in deciduous fruit trees, and gibberellins (GAs) are known to be particularly important. In this study, we observed that GA(4) treatment led to earlier bud break in Japanese apricot. To understand better the promoting effect of GA(4) on the dormancy release of Japanese apricot flower buds, proteomic and transcriptomic approaches were used to analyse the mechanisms of dormancy release following GA(4) treatment, based on two-dimensional gel electrophoresis (2-DE) and digital gene expression (DGE) profiling, respectively. More than 600 highly reproducible protein spots (P<0.05) were detected and, following GA(4) treatment, 38 protein spots showed more than a 2-fold difference in expression, and 32 protein spots were confidently identified according to the databases. Compared with water treatment, many proteins that were associated with energy metabolism and oxidation-reduction showed significant changes after GA(4) treatment, which might promote dormancy release. We observed that genes at the mRNA level associated with energy metabolism and oxidation-reduction also played an important role in this process. Analysis of the functions of the identified proteins and genes and the related metabolic pathways would provide a comprehensive proteomic and transcriptomic view of the coordination of dormancy release after GA(4) treatment in Japanese apricot flower buds.
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