Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 64, Issue 11, Pages 3285-3298Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ert168
Keywords
A; C-i curve fitting; chlorophyll fluorescence; Coffea arabica; Limonium gibertii; Nicotiana tabacum; variable J method
Categories
Funding
- National Council for Scientific and Technological Development (CNPq) [302605/2010-0]
- Plan Nacional (Spain) [AGL2009-07999]
- CNPq
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Mesophyll conductance (g(m)) can markedly limit photosynthetic CO2 assimilation and is required to estimate the parameters of the Farquharvon CaemmererBerry (FvCB) model properly. The variable J (electron transport rate) is the most frequently used method for estimating g(m), and the correct determination of J is one of its requirements. Recent evidence has shown that calibrating J can lead to some errors in estimating g(m), but to what extent the parameterization of the FvCB model is affected by calibrations is not well known. In addition to determining the FvCB parameters, variants of the J calibration method were tested to address whether varying CO2 or light levels, possible alternative electron sinks, or contrasting leaf structural properties might play a role in determining differences in , the product of the leaf absorptance () and the photosystem II optical cross-section (). It was shown that differences in were mainly attributed to the use of A/C-i or A/PPFD curves to calibrate J. The different values greatly influenced g(m), leading to a high number of unrealistic values in addition to affecting the estimates of the FvCB model parameters. A new approach was devised to retrieve leaf respiration in the light from combined A/C-i and A/C-c curves and a framework to understand the high variation in observed g(m) values. Overall, a background is provided to decrease the noise in g(m), facilitating data reporting and allowing better retrieval of the information presented in A/C-i and A/C-c curves.
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