Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 64, Issue 6, Pages 1755-1767Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ert040
Keywords
Arabidopsis; drought stress; freezing tolerance; gene regulation; lipid-transfer protein 3; MYB96
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Funding
- China National Funds for Distinguished Young Scientists [31225003]
- National Basic Research Program of China [2009CB119100]
- National Natural Science Foundation of China [31121002]
- Ministry of Agriculture of China for transgenic research [2011ZX08009-003-002]
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Several lipid-transfer proteins were reported to modulate the plant response to biotic stress; however, whether lipid-transfer proteins are also involved in abiotic stress remains unknown. This study characterized the function of a lipid-transfer protein, LTP3, during freezing and drought stress. LTP3 was expressed ubiquitously and the LTP3 protein was localized to the cytoplasm. A biochemical study showed that LTP3 was able to bind to lipids. Overexpression of LTP3 resulted in constitutively enhanced freezing tolerance without affecting the expression of CBFs and their target COR genes. Further analyses showed that LTP3 was positively regulated by MYB96 via the direct binding to the LTP3 promoter; consistently, transgenic plants overexpressing MYB96 exhibited enhanced freezing tolerance. This study also found that the loss-of-function mutant ltp3 was sensitive to drought stress, whereas overexpressing plants were drought tolerant, phenotypes reminiscent of myb96 mutant plants and MYB96-overexpressing plants. Taken together, these results demonstrate that LTP3 acts as a target of MYB96 to be involved in plant tolerance to freezing and drought stress.
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