Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 63, Issue 7, Pages 2739-2752Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/err456
Keywords
Alstroemeria; caryophyllene; gene expression; humulene; myrcene; scent emission; terpene synthase; volatiles
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Funding
- UK Biotechnology and Biological Sciences Research Council (BBSRC) [BB/G003572/1]
- School of Chemistry
- CONICYT, Ministry of Education, Government of Chile
- BBSRC [BB/G003572/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G003572/1] Funding Source: researchfish
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Native to South America, Alstroemeria flowers are known for their colourful tepals, and Alstroemeria hybrids are an important cut flower. However, in common with many commercial cut flowers, virtually all the commercial Alstroemeria hybrids are not scented. The cultivar 'Sweet Laura' is one of very few scented commercial Alstroemeria hybrids. Characterization of the volatile emission profile of these cut flowers revealed three major terpene compounds: (E)-caryophyllene, humulene (also known as alpha-caryophyllene), an ocimene-like compound, and several minor peaks, one of which was identified as myrcene. The profile is completely different from that of the parental scented species A. caryophyllaea. Volatile emission peaked at anthesis in both scented genotypes, coincident in cv. 'Sweet Laura' with the maximal expression of a putative terpene synthase gene AlstroTPS. This gene was preferentially expressed in floral tissues of both cv. 'Sweet Laura' and A. caryophyllaea. Characterization of the AlstroTPS gene structure from cv. 'Sweet Laura' placed it as a member of the class III terpene synthases, and the predicted 567 amino acid sequence placed it into the subfamily TPS-b. The conserved sequences R-28(R)X8W and (DDXXD)-D-321 are the putative Mg2+-binding sites, and in vitro assay of AlstroTPS expressed in Escherichia coli revealed that the encoded enzyme possesses myrcene synthase activity, consistent with a role for AlstroTPS in scent production in Alstroemeria cv. 'Sweet Laura' flowers.
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