Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 63, Issue 13, Pages 4731-4740Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ers141
Keywords
Deoxynivalenol; Fusarium head blight; trichothecenes; UDP-glucosyltransferase
Categories
Funding
- Austrian Science fund (SFB Fusarium) [F3701, F3706, F3708, L475-B09]
- Federal Ministry of Economy, Family, and Youth
- National Foundation for Research, Technology, and Development
- National Institute of Crop Science (NICS), Rural Development Administration (RDA), Republic of Korea
- Rural Development Administration, Republic of Korea [PJ00800602]
- USDA-ARS US Wheat and Barley Scab Initiative
- Minnesota Small Grains Initiative
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Fusarium head blight (FHB), caused by Fusarium graminearum, is a devastating disease of small grain cereal crops. FHB causes yield reductions and contamination of grain with trichothecene mycotoxins such as deoxynivalenol (DON). DON inhibits protein synthesis in eukaryotic cells and acts as a virulence factor during fungal pathogenesis, therefore resistance to DON is considered an important component of resistance against FHB. One mechanism of resistance to DON is conversion of DON to DON-3-O-glucoside (D3G). Previous studies showed that expression of the UDP-glucosyltransferase genes HvUGT13248 from barley and AtUGt73C5 (DOGT1) from Arabidopsis thaliana conferred DON resistance to yeast. Over-expression of AtUGt73C5 in Arabidopsis led to increased DON resistance of seedlings but also to dwarfing of transgenic plants due to the formation of brassinosteroid-glucosides. The objectives of this study were to develop transgenic Arabidopsis expressing HvUGT13248, to test for phenotypic changes in growth habit, and the response to DON. Transgenic lines that constitutively expressed the epitope-tagged HvUGT13248 protein exhibited increased resistance to DON in a seed germination assay and converted DON to D3G to a higher extent than the untransformed wild-type. By contrast to the over-expression of DOGT1 in Arabidopsis, which conjugated the brassinosteriod castasterone with a glucoside group resulting in a dwarf phenotype, expression of the barley HvUGT13248 gene did not lead to drastic morphological changes. Consistent with this observation, no castasterone-glucoside formation was detectable in yeast expressing the barley HvUGT13248 gene. This barley UGT is therefore a promising candidate for transgenic approaches aiming to increase DON and Fusarium resistance of crop plants without undesired collateral effects.
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