Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 59, Issue 12, Pages 3407-3414Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ern189
Keywords
diaminofluorescein; diethylamine NONOate; electrochemical sensor; nitric oxide; plant defence responses
Categories
Funding
- Conseil Re gional de Bourgogne [07 9201 CPER O2 S 5527]
- Agence Nationale de la Recherche [BLAN072-184783]
- Ministere de l'Education Nationale
- de la Recherche et de la Technologie
- L'Oreal France-UNESCO Academie des Sciences
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It was previously reported that cryptogein, an elicitor of defence responses, induces an intracellular production of nitric oxide (NO) in tobacco. Here, the possibility was explored that cryptogein might also trigger an increase of NO extracellular content through two distinct approaches, an indirect method using the NO probe 4,5-diaminofluorescein (DAF-2) and an electrochemical method involving a chemically modified microelectrode probing free NO in biological media. While the chemical nature of DAF-2-reactive compound(s) is still uncertain, the electrochemical modified microelectrodes provide real-time evidence that cryptogein induces an increase of extracellular NO. Direct measurement of free extracellular NO might offer important new insights into its role in plants challenged by biotic stresses.
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