Journal
JOURNAL OF EXPERIMENTAL BIOLOGY
Volume 216, Issue 4, Pages 543-553Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jeb.074757
Keywords
20S proteasome; Nrf2; Drosophila melanogaster; Caenorhabditis elegans; oxidative stress adapation; proteolysis
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Funding
- National Institutes of Health/National Institute of Environmental Health Sciences (NIH/NIEHS) [RO1-ES003598, 3RO1-ES 003598-22S2]
- Department of Health and Human Services [AG011833]
- National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS) [NS071085-02]
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In mammalian cells, hydrogen peroxide (H2O2)-induced adaptation to oxidative stress is strongly dependent on an Nrf2 transcription factor-mediated increase in the 20S proteasome. Here, we report that both Caenorhabditis elegans nematode worms and Drosophila melanogaster fruit flies are also capable of adapting to oxidative stress with H2O2 pre-treatment. As in mammalian cells, this adaptive response in worms and flies involves an increase in proteolytic activity and increased expression of the 20S proteasome, but not of the 26S proteasome. We also found that the increase in 20S proteasome expression in both worms and flies, as in mammalian cells, is important for the adaptive response, and that it is mediated by the SKN-1 and CNC-C orthologs of the mammalian Nrf2 transcription factor, respectively. These studies demonstrate that stress mechanisms operative in cell culture also apply in disparate intact organisms across a wide biological diversity.
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