Journal
JOURNAL OF ETHNOPHARMACOLOGY
Volume 144, Issue 1, Pages 190-194Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2012.09.003
Keywords
Laggera alata; Isochlorogenic acid A; Anti-hepatitis B virus; Hepatoprotective; Heme oxygenase-1
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Funding
- National Natural Science Foundation of China [30701049]
- opening foundation of the State Key Laboratory for Diagnosis and Treatment of Infectious Diseases
- The First Affiliated Hospital of Medical College
- Zhejiang University [2009KF10]
- Postdoctoral Science Research Project of Zhejiang Province [2011368]
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Ethnopharmacological relevance: The aim of this study was to determine the anti-hepatitis B effect of isochlorogenic acid A isolated from Laggera alata (Asteraceae), a traditional Chinese herbal medicine. Materials and methods: The anti-hepatitis B activity of isochlorogenic acid A was evaluated by the D-galactosamine (D-GalN)-induced HL-7702 hepatocyte damage model and the HBV-transfected HepG2.2.15 cells. Results: Isochlorogenic acid A significantly improved HL-7702 hepatocyte viability and markedly inhibited the productions of HBsAg and HBeAg. The inhibitory rates of isochlorogenic acid A on the HBsAg and HBeAg expressions were 86.9% and 72.9%, respectively. In addition, isochlorogenic acid A declined markedly the content of hepatitis B virus covalently closed circular DNA (HBV cccDNA) and induced significantly the heme oxygenase-1 (HO-1) expression in HepG2.2.15 cells. Conclusions: Isochlorogenic acid A was verified to possess the potent anti-hepatitis B activity. The anti-HBV target of isochlorogenic acid A is probably associated with blocking the translation step of the HBV replication. Overexpression of HO-1 may contribute to the anti-HBV activity of isochlorogenic acid A by reducing the stability of the HBV core protein and thus blocking the refill of nuclear HBV cccDNA. Additionally, the hepatoprotective effect of isochlorogenic acid A could be achieved by its antioxidative property and induction of HO-1. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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