Antinociceptive and anti-inflammatory kaempferol glycosides from Sedum dendroideum

Aim of the study: To identify the compounds responsible for the antinociceptive and anti-inflammatory effects previously described for Sedum dendroideum, through bioassay-guided fractionation procedures. Materials and methods: Antinociceptive activity was evaluated through mouse acetic acid-induced writhing model. The anti-inflammatory activity was assessed through croton oil-induced mouse ear oedema and carrageenan-induced peritonitis. Results: The Sedum dendroideum juice afforded seven known flavonoids identified with basis on NMR data. The oral administration of the major kaempferol glycosides kaempferitrin [1] (17.29 mu mol/kg), kaempferol 3-O-beta-glucopyranoside-7-O-alpha-rhamnopyranoside [2] (16.82 mu mol/kg), kaempferol 3-O-neohesperidoside-7-O-alpha-rhamnopyranoside [3] (13.50 mu mol/kg) or alpha-rhamnoisorobin [5] (23.13 mu mol/kg) inhibited by 47.3%, 25.7%, 60.2% and 58.0%, respectively, the acetic acid-induced nociception (indomethacin: 27.95 mu mol/kg, p.o.; 68.9%). Flavonoids 1, 2, 3 or 5, at the same doses, reduced by 39.5%,46.5%, 35.6% and 33.3%, respectively, the croton oil-induced oedema (dexamethasone: 5.09 mu mol/kg, s.c.; 83.7%) and impaired leukocyte migration by 42.9%,46.3%,50.4% and 49.6%, respectively (dexamethasone: 5.09 mu mol/kg, s.c.; 66.1 %). Conclusions: Our findings show that the major kaempferol glycosides may account for the renowned medicinal use of Sedum dendroideum against pain and inflammatory troubles. (C) 2009 Elsevier Ireland Ltd. All rights reserved.