Journal
JOURNAL OF ETHNOPHARMACOLOGY
Volume 124, Issue 1, Pages 39-44Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2009.04.020
Keywords
Cardiospermum halicacabum L.; Anti-inflammation; LPS; Cyclooxygenases; TNF-alpha; NF kappa B; iNOS; RAW264.7 cells; Jurkat T cells
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Funding
- CSIR New Delhi
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Aim of this study: Cardiospermum halicacabum L. is well known for its anti-inflammatory, analgesic and antipyretic activities. It has been used in Ayurveda and folk medicine for the treatment of rheumatism, fever and earache. But its mechanism of anti-inflammatory and analgesic action is still unclear, hence in this context, the objective of our study is to reveal the mechanism of anti-inflammatory and analgesic activity of Cardiospermum halicacabum L which would form an additional proof to the traditional knowledge of Cardiospermum halicacabum L. Materials and methods: In this study the ethanolic extract of the whole plant was used to evaluate the anti-inflammatory action in mouse macrophage cell line RAW264.7 cells. The expression levels of cyclooxygenase (COX)-1, COX-2. tumor necrosis factor-alpha (TNF-alpha), inducible nitric oxide synthase (iNOS) and COX-2 protein expression by semi-quantitative reverse transcriptase-polymerase chain reaction (RTPCR) and western blot and nuclear factor kappa-B (NF-kappa B) binding activity by electrophoretic mobility shift assay (EMSA). Results: We found that the ethanol extract close dependently inhibit mRNA expression of COX-2, TNF-alpha, iNOS and COX-2 protein expression. But the extract did not affect the expression of COX-1 mRNA expression. Furthermore, Cardiospermum halicacabum L ethanol extract inhibited the TNF-alpha induced DNA binding activity of NF-kappa B, which was associated with decreased p65 protein level in the nucleus in Jurkat cells. Conclusion: These results enabled to understand the mechanisms behind the anti-inflammatory and analgesic activity of Cardiospermum halicacabum L. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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