Journal
JOURNAL OF ETHNOPHARMACOLOGY
Volume 119, Issue 1, Pages 67-73Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2008.05.032
Keywords
Plumbago zeylanica; seselin; mononuclear cells; proliferation; IL-2; IFN-gamma
Categories
Funding
- National Science Council [NSC93-2320-B-030-001, NSC94-2320-B-030-001]
- Council of Agriculture [95C-0408]
- Taipei Veterans General Hospital [V95 E2-001, V96 E2-002, V97 E2-004]
- Fu-Jen University [9991A01-409531040114, 9991A15-109631040995-4]
- Republic of China
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Effects of seselin (C14H12O3; MW 228) identified from Plumbago zeylanica on phytohemagglutinin (PHA)-stimulated cell proliferation were studied in human peripheral blood mononuclear cells (PBMC). The data demonstrated that seselin inhibited PBMC proliferation-activated with PHA with an IC50 of 53.87 +/- 0.74 mu M. Cell viability test indicated that inhibitory effects of seselin on PBMC proliferation were not through direct cytotoxicity. The action mechanisms of seselin may involve the regulation of cell cycle progression, interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) production in PBMC. Since cell cycle analysis indicated that seselin arrested the cell cycle progression of activated PBMC from the G, transition to the S phase. Seselin suppressed IL-2 and IFN-gamma production in a concentration-dependent manner. Furthermore, seselin significantly decreased the IL-2 and IFN-gamma gene expression in PHA-activated PBMC. Therefore, results elucidated for the first time that seselin is likely an immunomodulatory agent for PBMC. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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