Journal
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
Volume 27, Issue 1, Pages 78-83Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/14756366.2011.576010
Keywords
Melanization; phenoloxidase; phenylthiourea; enzyme inhibition
Funding
- Russian Foundation of Basic Researches [09-04-01582, 10-04-01462]
- Sibirian branch of Russian Academy of Science [46]
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Phenoloxidase is a key enzyme of melanization catalyzing the oxidation of phenols. Phenylthiourea (PTU) is the well-known and widely used inhibitor of phenoloxidase. However, the mechanism of its action is not quite clear. In the present work, the effect of PTU on the enzymatic oxidation of 3-(3,4-dihydroxyphenyl)-L-alanine (DOPA) by phenoloxidase was studied by spectrophotometric methods. The inhibition constant of PTU was estimated as 0.21 +/- 0.09 mu M and the competitive type of inhibition was determined for this reaction.
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