Journal
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
Volume 25, Issue 6, Pages 798-803Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/14756360903476398
Keywords
Hinokitiol; tyrosinase; inhibition kinetics; melanin biosynthesis; antimicrobial activity
Funding
- National High Technology Research and Development Program (863 Program) of China [2006AA10A211]
- Natural Science Foundation of China [30570408, 20832005]
- Science and Technology Foundation of Fujian Province [2007N0051]
- Program for New Century Excellent Talents in Fujian Province University
- Postdoctoral Foundation of China [20080430787]
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The inhibitory effects of hinokitiol, a constituent of the woody oils isolated from Cupressaceae heartwood, on mushroom tyrosinase and melanin formation in B16 melanoma cells as well as its antimicrobial activity were investigated. Our results showed that hinokitiol could strongly inhibit both monophenolase activity and diphenolase activity of the enzyme and the inhibition was reversible. The IC50 values were estimated as 9.67 mu M for monophenolase activity and 0.21 mu M for diphenolase activity. The lag time of the monophenolase activity was not obviously lengthened by the compound. Kinetic analyses showed that the inhibition mechanism of hinokitiol was a mixed-type inhibition of the diphenolase activity. Hinokitiol effectively inhibited both cellular tyrosinase activity and melanin biosynthesis in B16 melanoma cells with significant cytotoxicity. Furthermore, it was found that hinokitiol could inhibit the proliferation of Salmonella enteritidis, Escherichia coli, Bacillus subtilis, Staphyloccocus aureus, Klebsiella pneumoniae, and Ralstonia solanacearum to different extents. This research may widen the use of hinokitiol in the fields of food preservation, depigmentation, and insecticide use.
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