Journal
JOURNAL OF ENVIRONMENTAL SCIENCES
Volume 23, Issue 4, Pages 649-655Publisher
SCIENCE PRESS
DOI: 10.1016/S1001-0742(10)60460-3
Keywords
cellulose-degrading bacterium; cellulose degradation capability; anaerobic bacterium; Clostridium
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Funding
- Key Projects in the National Science & Technology Pillar Program [2006BAD10B05-02, 2006BAD07A01]
- National Public Benefit Research Sector [200803033]
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An efficient cellulose degrading bacteria exists in the thermophilic wheat straw-degrading community, WDC2. However, this strain cannot 1312 isolated and cultured using conventional separation techniques under strict anaerobic conditions. We successfully isolated a strain cif effective cellulose degrading bacteria CTL-6 using a wash, heat shock, and solid-liquid alternating process. Analysis of its properties revealed that, although the community containing the strain CTL-6 grew under aerobic conditions, the purified strain CTL-6 only grew under anaerobic culture conditions. The strain CTL-6 had a striking capability of degrading cellulose (80.9% weight loss after 9 days of culture). The highest efficiency value of the endocellulase (CMCase activity) was 0.404 mu mol/(min.mL), cellulose degradation efficiency by CTL-6 was remarkably high at 50-65 degrees C with the highest degradation efficiency observed at 60 degrees C. The 16S rRNA gene sequence analysis indicated that the closest relative to strain CTL-6 belonged to the genus Clostridium thermocellum. Strain CTL-6 was capable of utilizing cellulose, cellobiose, and glucose. Strain CTL-6 also grew with Sorbitol as the sole carbon source, whereas C. thermocellum is unable to do so.
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