Journal
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
Volume 49, Issue 4, Pages 305-311Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/03601234.2014.868683
Keywords
Acetic acid; acid hydrolysis; bioethanol; furfural; hydroxymethyl furfural; Saccharomyces cerevisiae; soybean meal; Zymomonas mobilis
Funding
- Arkansas Soybean Promotion Board
- COLCIENCIAS of Colombia
- LASPAU
- Universidad de Cordoba (Colombia)
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The objective of this research was to determine the minimum inhibitory concentration of 5-hydroxymethyl furfural, furfural, and acetic acid on Saccharomyces cerevisiae (NRRL Y-2233) and Zymomonas mobilis subspecies mobilis (NRRL B-4286) in both detoxified hydrolyzed soybeanmeal and synthetic YM broth spiked with the three compounds. Soybean meal was hydrolyzed with dilute sulfuric acid (0.0, 0.5, 1.25, and 2.0% wt v(-1)) at three temperatures (105, 120, and 135 degrees C) and three durations (15, 30, and 45 min) followed by detoxification with activated carbon. Of all the combinations, only the treatments obtained at 135 degrees C, 2.0% H2SO4, and 45 min and the one at 135 degrees C, 1.25% H2SO4, and 45 min showed inhibition in the growth of the tested microorganisms. Spiked YM broths showed inhibition for the highest levels of inhibitors, either applied individually or in combination.
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