Journal
JOURNAL OF ENDODONTICS
Volume 38, Issue 2, Pages 170-176Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2011.10.010
Keywords
Adenovirus; bone morphogenetic protein-7; dental pulp stem cell; gene therapy; regenerative endodontics
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Funding
- Natural Science Foundation of China [81000679]
- Fundamental Research Funds for the Central Universities of China [3082026]
- Youth Chenguang Project of Science and Technology of Wuhan City, China [201150431106]
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Introduction: Recombinant human bone morphogenetic protein-7 (BMP-7) is a well-established agent to induce bone and dentin formation. Little is understood until now whether BMP-7 could be used to genetically modify human dental pulp stem cells for tissue engineering applications. Methods: This study was to determine the feasibility of mineralized tissue formation from human dental pulp-derived stem cells (DPSCs) transfected with adenoviral-mediated human BMP-7 gene through in vitro and in vivo evaluations. Results: In vitro results of alkaline phosphatase, calcium content, and real-time polymerase chain reaction showed that BMP-7 transfected cells had the ability to differentiate towards the odontoblast phenotype and produce a calcified extracellular matrix. Transfected cell were seeded onto a porous ceramic scaffold and implanted subcutaneously in mice. Samples were retrieved after 4 and 8 weeks for histology evaluation. The results indicated that only the cultures with BMP-7 gene transfection showed obvious hard-tissue generation. Conclusions: Our data suggest that adenovirus-mediated BMP-7 expression can induce odontogenic differentiation of human DPSCs and show effectively mineralized tissue formation in vivo, which may provide support for gene therapy candidate of BMP-7 in dental tissue engineering. (J Endod 2012;38:170-176)
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