Journal
JOURNAL OF ENDODONTICS
Volume 36, Issue 5, Pages 894-898Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2010.01.002
Keywords
Enterococcus faecalis; ethylenediaminetetraacetic acid; root canal irrigants; sodium hypochlorite
Categories
Funding
- Conselho Nacional de Pesquisa (CNPq)
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)
- CNPq-Brazil
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Introduction: The elimination of microorganisms from root canals is a critical step in endodontic treatment. We aimed to evaluate the antimicrobial effectiveness of an alternating irrigation regimen during chemomechanical preparation (CMP). Methods: During 21 days, root canals of extracted human teeth were infected with Enterococcus faecalis, and colonization was confirmed by scanning electron microscopy (SEM). Canals were irrigated with saline solution (control group), with 5.25% NaOCl followed by a final rinse with 17% EDTA (conventional irrigation group), or with the alternating use of NaOCl and EDTA (alternating irrigation [Al] group). Samples were taken before treatment (S1), after CMP (S2), and during the following 14 days. Two specimens/group were analyzed by SEM. Results: The Al group yielded negative agar and liquid cultures from immediately after CMP and from the 5th day on, respectively. SEM confirmed several bacterium-free sites in the Al group. Conclusion: The irrigation regimen based on the alternating use of NaOCl and EDTA seems to be a promising endodontic tool because it promoted the elimination of root canal E. faecalis biofilms throughout the experimental period. (J Ended 2010;36:894-898)
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