Journal
JOURNAL OF ENDODONTICS
Volume 35, Issue 6, Pages 858-865Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2009.03.049
Keywords
Dentin defect; fibroblast growth factor-2; gelatin hydrogels; pulp exposure; regeneration
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Funding
- Ministry of Education, Science, and Culture of Japan, Tokyo, Japan [18209057, 20592231]
- Grants-in-Aid for Scientific Research [18209057, 20592231] Funding Source: KAKEN
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Introduction: Pulp regeneration therapy is important to overcome the limitations of conventional therapy to induce reparative dentinogenesis. In the present study, we examined the effects of controlled release of different dosages of fibroblast growth factor-2 (FGF-2) from gelatin hydrogels to regenerate the dentin-pulp complex. Methods: After the amputation of dental pulp of rat molars, gelatin hydrogels incorporating various dosages of FGF-2 were individually implanted into dentin defects above the sites of the amputated pulps. Histologic changes as well as the expression of dentin matrix protein-1 (DMP-1) and nestin in the dentin defect area above the amputated pulp were analyzed. Results: We found that controlled release of high doses of FGF-2 from gelatin hydrogels induced DMP-1-positive calcified particles in the proliferating pulp, whereas a moderate dose of FGF-2 induced DMP-1-positive dentinal bridge on the surface of the proliferating pulp. These findings indicate that the dosage of released FGF-2 has an influence on the structure of calcified tissue regenerated in dentin defects. In addition, pulp cells near calcified tissues regenerated in dentin defects were nestin-negative, suggesting that the calcified tissues might be osteodentin, Conclusions: Our results showed that the dentin regeneration on amputated pulp, not reparative dentin formation toward amputated pulp, can be regulated by adjusting the dosage of FGF-2 incorporated in biodegradable gelatin hydrogels. (J Endod 2009;35:858-865)
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