Optimal Cytocompatibility of a Bioceramic Nanoparticulate Cement in Primary Human Mesenchymal Cells

Introduction: The aim of this study was to evaluate and compare the cytotoxic effects of BioAggregate (Innovative BioCaramix Inc, Vancouver, BC, Canada), a novel bioceramic nanoparticulate cement, on human mesenchymal cells. White Pro-Root MTA (Dentsply, Tulsa Dental, Tulsa, OK) was used as a reference for comparison. Methods: Fifty-six human maxillary incisor teeth were submitted to a step-back flaring technique and prepared for cytotoxicity assay in an in situ root-end filling experimental model. After retro filling, each root containing MTA, BioAggregate, or empty root canals (control) was exposed to culture media for 24, 48, or 72 hours, providing several extraction media. Mesenchymal cells were incubated with each extract medium for 24 hours, and toxicity was evaluated by three different parameters of cell survival and integrity on the same sample: XTT, neutral red, and crystal violet dye elution. Results: No statistically significant differences between MTA and BioAggregate were found in all the experimental periods (p > 0.05). Conclusion: DiaRoot BioAggregate displayed in vitro compatibility similar to MTA. (J Endod 2009,35:1387-1390)