Journal
JOURNAL OF ENDODONTICS
Volume 35, Issue 6, Pages 847-851Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2009.03.014
Keywords
Enamel matrix derivative; human dental pulp cell; mineral trioxide aggregate; mineralization; odontoblastic differentiation
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Introduction: The current study investigated the effectiveness of enamel matrix derivative (EMD) and mineral trioxide aggregate (MTA) in inducing the differentiation of human dental pulp cells (HDPCs) into odontoblast-like cells in vitro. Methods: The effects of MTA and EMD on odontoblastic differentiation were indexed by alkaline phosphatase (ALP) activity and the expression of odontoblastic/osteoblastic markers, as determined by reverse-transcription polymerase chain reaction analysis. Mineralization was also evaluated by the staining of calcium deposits with Alizarin red. Cells were treated with a combination of MTA and EMD or with MTA alone. Results: Compared with MTA-treated cells on day 3, MTA/EMD-treated cells exhibited significantly greater increases in ALP activity and in dentin sialophosphoprotein and bone sialoprotein expression. Mineralization was significantly greater on day 7 in MTA/EMD-treated cells than in MTA-treated cells. Conclusions: These results suggest that a combination of MTA and EMD promotes more rapid differentiation in HDPCs than MTA alone and that this combination is thus a potential pulp-capping agent. (J Endod 2009;35:847-851)
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