Journal
JOURNAL OF ENDODONTICS
Volume 34, Issue 11, Pages 1332-1336Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2008.08.021
Keywords
Acute apical abscess; apical periodontitis; clonal typing; polymerase chain reaction; Porphyromonas gingivalis; 16S rRNA gene
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This study investigated the prevalence of Porphyromonas gingivalis in 62 teeth with primary endodontic infections by using a species-specific 16S rRNA gene-based nested polymerase chain reaction assay. P. gingivalis isolates recovered from 2 infected root canals were also analyzed for clonal diversity by using arbitrarily primed PCR. Overall, P. gingivalis was found in 48% of the samples. This species was specifically detected in 36% of canals of teeth with chronic apical periodontitis, in 46% of the cases of acute apical periodontitis, and in 67% of acute apical abscesses. P. gingivalis was significantly more frequent in abscess aspirates than in canals of teeth with chronic apical periodontitis (P<.05). Typing of colonies retrieved from 2 infected canals revealed 2 clones per individual. These findings confirmed that P. gingivalis can be an important endodontic pathogen, mostly associated with acute abscesses, and demonstrated that different clonal types of this species can colonize the root canal in the same individual. (J Endod 2008;34:1332-1336)
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