Journal
JOURNAL OF ENDOCRINOLOGY
Volume 216, Issue 3, Pages 331-341Publisher
BIOSCIENTIFICA LTD
DOI: 10.1530/JOE-12-0486
Keywords
17 beta-estradiol; C2C12 skeletal myoblasts; antiapoptotic effect; MPTP; MnSOD
Categories
Funding
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
- Universidad Nacional del Sur, Argentina
Ask authors/readers for more resources
17 beta-Estradiol (E-2) protects several non-reproductive tissues from apoptosis, including skeletal muscle. We have shown that E-2 at physiological concentrations prevented apoptosis induced by H2O2 in C2C12 skeletal myoblasts. As we also demonstrated the presence of estrogen receptors in mitochondria, the present work was focused on the effects of E-2 on this organelle. Specifically, we evaluated the actions of E-2 on the mitochondrial permeability transition pore (MPTP) by the calcein-acetoxymethylester/cobalt method using fluorescence microscopy and flow cytometry. Pretreatment with E-2 prevented MPTP opening induced by H2O2, which preceded loss of mitochondrial membrane potential. In addition, it was observed that H2O2 induced translocation of Bax to mitochondria; however, in the presence of the steroid this effect was abrogated suggesting that members of the Bcl-2 family may be regulated by E-2 to exert an antiapoptotic effect. Moreover, E-2 increased mitochondrial manganese superoxide dismutase protein expression and activity, as part of a mechanism activated by E-2 that improved mitochondrial performance. Our results suggest a role of E-2 in the regulation of apoptosis with a clear action at the mitochondrial level in C2C12 skeletal myoblast cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available