Journal
JOURNAL OF ENDOCRINOLOGY
Volume 198, Issue 3, Pages 533-540Publisher
BIOSCIENTIFICA LTD
DOI: 10.1677/JOE-08-0105
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Funding
- NHMRC Australia
- Eli Lilly Endocrinology Program
- Shaanxi International Cooperation [2005KW-17]
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ATP-sensitive potassium channels (K-ATP channels) determine the excitability of pancreatic beta-cells and importantly regulate glucose-stimulated insulin secretion (GSIS). Long-chain free fatty acids (FFAs) decrease GSIS after long-term exposure to beta-cells, but the effects of exogenous FFAs on K-ATP channels are not yet well clarified. In this study, the effects of linoleic acid (LA) on membrane potential (MP) and K-ATP channels were observed in primary cultured rat pancreatic beta-cells. LA (20 mu M) induced hyperpolarization of MP and opening of K-ATP channels, which was totally reversed and inhibited by tolbutamide, a K-ATP channel blocker. Inhibition of LA metabolism by acyl-CoA synthetase inhibitor, triacsin C (10 mu M), partially inhibited LA-induced opening of K-ATP channels by 64%, The non-FFA G protein-coupled receptor (GPR) 40 agonist, GW9508 (40 mu M), induced an opening of K-ATP channels, which was similar to that induced by LA under triacsin C treatment. Blockade of protein kinases A and C did not influence the opening of K-ATP channels induced by LA and GW9508, indicating that these two protein kinase pathways are not involved in the action of LA on K-ATP channels. The present study demonstrates that LA induces hyperpolarization of MP by activating K-ATP channels via both intracellular metabolites and activation of GPR40. It indicates that not only intracellular metabolites of FFAs but also GPR40-mediated pathways take part in the inhibition of GSIS and beta-cell dysfunction induced by FFAs.
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