Advanced oxidation protein products are more related to metabolic syndrome components than biomarkers of lipid peroxidation

Although advanced oxidation protein products (AOPPs) have been reported as the most appropriate parameter for determination of oxidative stress in patients with metabolic syndrome (MetS), a direct comparison between protein and lipid peroxidation has not been performed yet. The aim of this study was to compare protein peroxidation with lipid peroxidation measured by 2 different methodologies (tert-butyl hydroperoxide-initiated chemiluminescence and ferrous oxidation-xylenol orange assay). The hypothesis of this study was that AOPPs would be more related to MetS than to oxidative markers of lipid peroxidation. This cross-sectional study evaluated 76 patients with MetS and 20 healthy subjects. Prooxidant-antioxidant index (PAI) assessed as AOPP/total radical-trapping antioxidant parameter ratio progressively increased (P<.05) according to the number of MetS components, whereas AOPPs and total radical-trapping antioxidant parameter increased (P<.05) when 5 components were compared with 3 components. Spearman test showed a positive correlation between AOPPs and waist circumference (r = 0.318, P<.01), fasting glucose (r = 0.250, P<.05), homeostasis model assessment insulin resistance (r = 0.043, P<.01), triacylglycerol (r = 0.713, P<.0001), highly sensitive C-reactive protein (r = 0.275, P<.05), and uric acid (r = 0.356, P<.01), whereas there was an inverse correlation with high-density lipoprotein cholesterol (r = -0.399, P<.001). Prooxidant-antioxidant index demonstrated a positive correlation with waist circumference (r = 0.386, P<.01), fasting glucose (r = 0.388, P<.01), fasting insulin (r = 0.344, P<.05), homeostasis model assessment insulin resistance (r = 0.519, P<.001), triacylglycerol (r = 0.687, P<.0001), highly sensitive C-reactive protein (r = 0.278, P<.05), and uric acid (r = 0.557, P<.0001), whereas there was an inverse correlation with high-density lipoprotein cholesterol (r = -0.480, P<.0001). In conclusion, protein peroxidation determined by AOPPs, and especially by PAL is more related to MetS components than lipid peroxidation. In addition, PAI progressively increased with the number of MetS components. (C) 2015 Elsevier Inc. All rights reserved.