Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 824, Issue -, Pages 201-206Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2018.07.053
Keywords
DNA probe; Mercury ions; Au nanoparticles; Reduced graphene oxide
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Funding
- Natural Science Foundation of Shandong Province, China [ZR2018BC055]
- PhD Startup Foundation of Shandong University of Technology, China [4041-417016]
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The present work describes an electrochemical Hg2+ ion biosensor which used Au nanoparticles decorated reduced graphene oxide (Au NPs-RGO) for generating electrochemical signal as indicator involving. Hg2+ ions specifically captured two thymine bases (T) of DNA probe to form T-Hg2+-T stable base pairs, therefore, Hg2+ ion solution induced the structure of Au NPs-RGO modified thiol-functionalized DNA probe from single-strand to hairpin. The DNA 1 modified on Au electrode is complementary with Au NPs-RGO modified single-stranded DNA probe. Therefore, the amount of Au NPs-RGO adsorbed on electrode is decreased when the concentrations of Hg2+ ions increase. The current response of the fabricated biosensor is monitored on ferricyanide system, which exhibits a wide linear range of 0-2000 nM and a high selectivity towards Hg2+ ion. Besides, there are no co-factors used in the process of fabricating biosensor. Moreover, the biosensor is applied for detecting Hg2+ ions in water sample with satisfactory results.
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