Journal
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 661, Issue 1, Pages 8-12Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2011.06.024
Keywords
L-Lactate; Biosensors; Electrochemical method; ZnO nanoparticles; CNTs
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Funding
- National Natural Science Foundation of China [60976004]
- Shanghai Municipal Commission for Science and Technology [09JC1405300]
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A highly sensitive and stable I.-lactate sensor based on the synergic action of multi-wall carbon nanotube (MWCNTs) and ZnO nanoparticles has been developed. The unique sandwich-like layer structure (PDDA/LOD/ZnO/MWCNTs) provides a favorable microenvironment to keep the bioactivity of LOD and prevent enzyme molecule leakage. Therefore, the proposed L.-lactate biosensor exhibited good analytical performances including high sensitivity and selectivity with satisfactory stability to amperometric determination of L-lactate. The results indicated that the proposed PDDA/LOD/ZnO/MWCNTs film was an attractive matrix for the immobilization of LOD enzymes to fabricate biosensors. (C) 2011 Elsevier B.V. All rights reserved.
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