4.7 Article

Polyelectrostatic immobilization of gold nanoparticles-modified peroxidase on alginate-coated gold electrode for mediatorless biosensor construction

Journal

JOURNAL OF ELECTROANALYTICAL CHEMISTRY
Volume 629, Issue 1-2, Pages 126-132

Publisher

ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2009.02.004

Keywords

Biosensor; Nanoparticle; Enzyme immobilization; Peroxidase; Sodium alginate

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Horseradish peroxidase was cross-linked with cysteamine-capped Au nanoparticles and further immobilized on sodium alginate-coated Au electrode through polyelectrostatic interactions. The electrode was employed for constructing a reagentless amperometric biosensor for H2O2. The electrode showed linear response (poised at -400 mV vs. Ag/AgCl) toward H2O2 concentration between 20 mu M and 13.7 mM at pH 7.0. The biosensor reached 95% of steady-state current in about 15 s, and its sensitivity was 40.1 mA/M cm(2). The detection limit of the enzyme-based electrode was determined as 3 mu M, at a signal-to-noise ratio of three. The electrode retained 97% of its initial analytical response after 1 month of storage at 4 degrees C in 50 mM sodium phosphate buffer, pH 7.0. The stability of the biosensor was significantly reduced when it was incubated in high ionic strength solutions, retaining only 44% of its initial response after 1 month of storage at 4 degrees C in 1 M NaCl ionic strength in 50 mM sodium phosphate buffer, pH 7.0. (c) 2009 Elsevier B.V. All rights reserved.

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