Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 178, Issue 8, Pages 1471-1480Publisher
SPRINGER
DOI: 10.1007/s12010-015-1960-0
Keywords
Esterase; Bacillus cereus; Enantioselective hydrolysis; Recombinant; alpha-ethyl-2-oxo-pyrrolidineacetic acid methyl ester
Funding
- National Nature Science Foundation of China [21202150]
- Natural Science Foundation of Zhejiang Province [Y4110468]
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A new esterase-producing strain (Bacillus cereus WZZ001) which exhibiting high hydrolytic activity and excellent enantioselectivity on rac-alpha-ethyl-2-oxo-pyrrolidineacetic acid methyl ester (R, S-1) has been isolated from soil sample by our laboratory. In this study, the stereoselective hydrolysis of (R, S-1) was performed using the recombinant Bacillus cereus esterase which expressed in Escherichia coli BL21 (DE3). Under the optimized conditions of pH 8.0, 35 A degrees C, and concentration of substrate 400 mM, a successful enzymatic resolution was achieved with an e.e. (s) of 99.5 % and conversion of 49 %. Immobilization considerably increased the reusability of the recombinant esterase; the immobilized enzyme showed excellent reusability during 6 cycles of repeated 2 h reactions at 35 A degrees C. Thereby, it makes the recombinant B. cereus esterase a usable biocatalyst for industrial application.
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