Journal
NUCLEIC ACIDS RESEARCH
Volume 44, Issue 4, Pages 1944-1951Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkv1517
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Funding
- Deutsche Forschungsgemeinschaft [SFB646, WI3285/3-1, GRK 1721, FOR1805]
- European Research Council
- Boehringer Ingelheim Fonds Fellowship
- DFG Fellowship through the Graduate School of Quantitative Biosciences Munich (QBM)
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During protein synthesis, ribosomes become stalled on polyproline-containing sequences, unless they are rescued in archaea and eukaryotes by the initiation factor 5A (a/eIF-5A) and in bacteria by the homologous protein EF-P. While a structure of EF-P bound to the 70S ribosome exists, structural insight into eIF-5A on the 80S ribosome has been lacking. Here we present a cryo-electron microscopy reconstruction of eIF-5A bound to the yeast 80S ribosome at 3.9 angstrom resolution. The structure reveals that the unique and functionally essential post-translational hypusine modification reaches toward the peptidyltransferase center of the ribosome, where the hypusine moiety contacts A76 of the CCA-end of the P-site tRNA. These findings would support a model whereby eIF-5A stimulates peptide bond formation on polyproline-stalled ribosomes by stabilizing and orienting the CCA-end of the P-tRNA, rather than by directly contributing to the catalysis.
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