Journal
NUCLEIC ACIDS RESEARCH
Volume 44, Issue 2, Pages 910-916Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkv1357
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Funding
- Singapore Ministry of Education Academic Research Fund Tier 3 [MOE2012-T3-1-001]
- Nanyang Technological University
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G- quadruplexes are non- canonical structures of nucleic acids, in which guanine bases form planar Gtetrads ( G G G G) that stack on each other in the core of the structure. G- quadruplexes generally contain multiple times of four ( 4n) guanines in the core. Here, we study the structure of G- quadruplexes with only ( 4n - 1) guanines in the core. The solution structure of a DNA sequence containing 11 guanines showed the formation of a parallel G- quadruplex involving two Gtetrads and one G- triad with a vacant site. Molecular dynamics simulation established the formation of a stable G- triad water complex, where water molecules mimic the position of the missing guanine in the vacant site. The concept of forming G- quadruplexes with missing guanines in the core broadens the current definition of G- quadruplex- forming sequences. The potential ability of such structures to bind different metabolites, including guanine, guanosine and GTP, in the vacant site, could have biological implications in regulatory functions. Formation of this unique binding pocket in the G- triad could be used as a specific target in drug design.
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