Journal
NUCLEIC ACIDS RESEARCH
Volume 43, Issue 14, Pages 6714-6729Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkv606
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Funding
- King Abdullah University of Science and Technology
- European Research Council [ERC] [RNA+P = 123D]
- King Abdullah University of Science and Technology (KAUST)
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Posttranscriptional modifications greatly enhance the chemical information of RNA molecules, contributing to explain the diversity of their structures and functions. A significant fraction of RNA experimental structures available to date present modified nucleobases, with half of them being involved in H-bonding interactions with other bases, i.e. 'modified base pairs'. Herein we present a systematic investigation of modified base pairs, in the context of experimental RNA structures. To this end, we first compiled an atlas of experimentally observed modified base pairs, for which we recorded occurrences and structural context. Then, for each base pair, we selected a representative for subsequent quantum mechanics calculations, to find out its optimal geometry and interaction energy. Our structural analyses show that most of the modified base pairs are non Watson-Crick like and are involved in RNA tertiary structure motifs. In addition, quantum mechanics calculations quantify and provide a rationale for the impact of the different modifications on the geometry and stability of the base pairs they participate in.
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