Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 177, Issue 5, Pages 1083-1098Publisher
SPRINGER
DOI: 10.1007/s12010-015-1798-5
Keywords
Inulin hydrolyzing enzyme; Trichosporon cutaneum; Dry cell weight; Lipid production; Consolidated bioprocessing
Funding
- National Basic Research Program of China [2011CB707406]
- National High-Tech Program of China [2012AA022301, 2014AA021901]
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Oleaginous yeast Trichosporon cutaneum CGMCC 2.1374 was found to utilize inulin directly for microbial lipid fermentation without a hydrolysis step. The potential inulinase-like enzyme(s) in T. cutaneum CGMCC 2.1374 were characterized and compared with other inulinase enzymes produced by varied yeast strains. The consolidated bioprocessing (CBP) for lipid accumulated using inulin was optimized with 4.79 g/L of lipid produced from 50 g/L inulin with the lipid content of 33.6 % in dry cells. The molecular weight of the enzyme was measured which was close to invertase in Saccharomyces cerevisiae. The study provided information for inulin hydrolyzing enzyme(s) in oleaginous yeasts, as well as a preliminary CBP process for lipid production from inulin feedstock.
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