Journal
JOURNAL OF DERMATOLOGICAL SCIENCE
Volume 56, Issue 1, Pages 33-36Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jdermsci.2009.06.010
Keywords
Perforin; T cell; Methylation; Systemic lupus erythematosus; Subacute cutaneous lupus erythematosus
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Funding
- National Natural Science Foundation of China [30730083, 30671883]
- National Basic Research Program of China (973 Plan) [2009CB825605]
- Hunan Natural Science Foundation [06C0049]
- Science Foundation of Hunan Province [06SK3033]
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Background: Recent evidence indicates that human lupus is an epigenetic disease characterized by impaired T cell DNA methylation. Perforin, a cytotoxic effector molecule, is overexpressed due to hypomethylation of its promoter regulatory elements in CD4(+) T cells from patients with systemic erythematosus lupus (SLE). However, it is unknown whether aberrant expression and methylation of perforin occur in CD4(+) T cells from patients with subacute cutaneous lupus erythematosus (SCLE). Objective: We aimed to compare the perforin expression level and the methylation status of the perforin promoter region in CD4(+) T cells from SCLE patients and healthy controls. Methods: We used real-time RT-PCR to compare the perforin mRNA levels, and Western-blot to compare perforin protein levels in CD4(+) and CD8(+) T cells from SCLE patients and healthy controls. Bisulfite sequencing was used to determine the methylation status of the perforin promoter region. Results: Perforin is overexpressed in SCLE CD4(+) T cells. Demethylation of the perforin promoter region was seen in CD4(+) T cells from patients with SCLE. Conclusions: DNA demethylation at the perforin locus contributes to perforin overexpression in SCLE CD4(+) T cells. (C) 2009 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.
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