Effects of Chitosan Particles in Periodontal Pathogens and Gingival Fibroblasts

Chitosan is a naturally derived polymer with antimicrobial and anti-inflammatory properties. However, studies evaluating the role of chitosan in the control of periodontal pathogens and the responses of fibroblasts to inflammatory stimuli are lacking. In the present study, we analyzed whether chitosan particles may inhibit the growth of periodontal pathogens and modulate the inflammatory response in human gingival fibroblasts. Chitosan particles were generated through ionic gelation. They inhibited the growth of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans at 5 mg/mL. Conversely, IL-1 strongly stimulated PGE(2) protein levels in gingival fibroblasts, and chitosan inhibited this response at 50 mu g/mL. IL-1-stimulated PGE(2) production was dependent on the JNK pathway, and chitosan strongly inhibited this response. IL-1 stimulated NF-B activation, another signaling pathway involved in PGE(2) production. However, chitosan particles were unable to modify NF-B signaling. The present study shows that chitosan exerts a predominantly anti-inflammatory activity by modulating PGE(2) levels through the JNK pathway, which may be useful in the prevention or treatment of periodontal inflammation.