Journal
JOURNAL OF DENTAL RESEARCH
Volume 91, Issue 12, Pages 1119-1123Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/0022034512455801
Keywords
dentin; tooth erosion; matrix metalloproteinases; extracellular matrix; hydroxyproline; enzyme inhibitors
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Funding
- FAPESP [07/04209-0, 07/08389-3]
- CNPq [557863/2008-2]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [07/08389-3] Funding Source: FAPESP
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This proof-of-concept study assessed whether the reduction of the degradation of the demineralized organic matrix (DOM) by pre-treatment with protease inhibitors (PI) is effective against dentin matrix loss. Bovine dentin slices were demineralized with 0.87 M citric acid, pH 2.3, for 36 hrs. In sequence, specimens were treated or not (UT, untreated) for 1 min with gels containing epigallocatechin 3-gallate (EGCG, 400 A mu M), chlorhexidine (CHX, 0.012%), FeSO4 (1 mM), NaF (1.23%), or no active compound (P, placebo). Specimens were then stored in artificial saliva (5 days, 37 degrees C) with the addition of collagenase (Clostridium histolyticum, 100 U/mL). We analyzed collagen degradation by assaying hydroxyproline (HYP) in the incubation solutions (n = 5) and evaluated the dentin matrix loss by profilometry (n = 12). Data were analyzed by ANOVA and Tukey's test (p < 0.05). Treatment with gels containing EGCG, CHX, or FeSO4 led to significantly lower HYP concentrations in solution and dentin matrix loss when compared with the other treatments. These results strongly suggest that the preventive effects of the PI tested against dentin erosion are due to their ability to reduce the degradation of the DOM.
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