Journal
JOURNAL OF DAIRY SCIENCE
Volume 97, Issue 11, Pages 6693-6699Publisher
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2014-7990
Keywords
frescal (fresh) goat cheese; bovine and caprine species-specific PCR; mitochondrial DNA; consumer detection threshold
Funding
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Rio de Janeiro, Brazil)
- FAPERJ (Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro, Rio de Janeiro, Brazil)
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The Brazilian market for dairy products made from goat milk is increasing despite the seasonality of production and naturally small milk production per animal, factors that result in high-priced products and encourage fraud. In Brazil, no official analytical method exists for detecting adulteration of goat dairy products with cow milk. The aim of this study was to design a strategy to investigate the adulteration of frescal (fresh) goat cheeses available in the Rio de Janeiro retail market, combining analysis of cheese composition and the perception of adulteration by consumers. Commercial goat cheeses were tested by using a duplex PCR assay previously designed to authenticate cheeses, by targeting the mitochondrial 12S ribosomal RNA genes of both species simultaneously. The PCR test was able to detect 0.5% (vol/vol) cow milk added during goat cheese formulation. The analysis of 20 locally produced goat cheeses (20 lots of 4 brands) showed that all were adulterated with cow milk, even though the labels did not indicate the addition of cow milk. To estimate the ability of consumers to perceive the fraudulent addition of cow milk, a triangle test was performed, in which cheeses formulated with several different proportions of goat and cow milk were offered to 102 regular consumers of cheese. Detection threshold analysis indicated that almost half of the consumers were able to perceive adulteration at 10% (vol/vol) cow milk. Effective actions must be implemented to regulate the market for goat dairy products in Brazil, considering the rights and choices of consumers with respect to their particular requirements for diet and health, preference, and cost.
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