High-pressure processing inactivates Listeria innocua yet compromises Queso Fresco crumbling properties

The objective of this study was to determine the effectiveness of high-pressure processing to inactivate Listeria innocua (a Listeria rnonocytogenes surrogate) in Queso Fresco, and to study the effects of the high-pressure treatment on cheese-crumbling properties. Queso Fresco was made with pasteurized, homogenized milk, lactic acid bacterial starter culture, chymosin, and flake salt. Cheeses were pressed (0.1 MPa) for 1 h before crumbling and inoculation with a cocktail of 3 strains of L. innocua, and then pressed for 12 h (0.1 MPa). High-pressure processing treatments of sliced cheese rounds included pressure from 400 to 600 MPa for 1 to 25 min Cheese sample temperatures, initially approximately 21 degrees C, increased during pressurization and decreased gradually during the holding time. The highest temperature increase was to 23.6 degrees C at 600 MPa. Greater than 5-log reductions occurred at set-point pressures of 500, 550, or 600 MPa when held for at least 15, 3, or 1 min, respectively. However, because inactivation was neither complete nor permanent and crumbling properties were not maintained under the conditions tested in this study, high-pressure processing is not recommended for Queso Fresco applications.