Journal
JOURNAL OF DAIRY SCIENCE
Volume 95, Issue 3, Pages 1410-1418Publisher
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2011-4532
Keywords
colostrum quality; immunoglobulin G; near-infrared spectroscopy; transflectance
Funding
- CONICYT (Chilean National Commission for Scientific and Technological Research)
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The objective of this work was to assess the potential of near infrared spectroscopy to predict the immunoglobulin G (IgG) content in bovine colostrum. Liquid colostrum samples (n = 157) were collected from Holstein cows from 2 dairy farms in southern Chile. Samples were obtained within 1 h of parturition and scanned in folded transmission (transflectance) in the visible-near infrared range. Multivariate regression models (modified partial least squares) were developed with spectral data against IgG content measured by radial immunodiffusion. The best calibration included a mathematical treatment of the spectra by a second derivative plus standard normal variate and detrending. The best equation explained a high proportion of the variation in IgG content (R-2 of 0.95 in calibration and 0.94 in cross-validation). Average (91.5 g/L), standard deviation (37.6 g/L), and range, as highest minus lowest values (171.9 g/L) of reference values were 10.1, 4.2, and 19 times the value of the root mean square error of cross-validation (9.03 g/L) respectively. Near-infrared spectroscopy, scanned in folded transmission, is an effective tool to predict the IgG content in liquid colostrum.
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