Journal
JOURNAL OF DAIRY SCIENCE
Volume 95, Issue 4, Pages 1639-1644Publisher
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2011-4734
Keywords
coagulase-positive staphylococci; Staphylococcus aureus; sheep milk; coa gene
Funding
- Junta de Castilla y Leon (Ayuda Grupo de Excelencia) [GR155]
- CONSOLIDER - ingenio
- CARNISENUSA [CSD2007-00016]
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The prevalence of coagulase-positive staphylococci (CPS) was studied among 390 samples of ewe milk. Fifty-seven (14.85%) samples of tank milk and all samples (6) of silo milk gave a positive result on Baird-Parker agar base supplemented with rabbit plasma fibrinogen, whereas amplification of the coagulase (coa) gene was successful in 6 (1.56%) samples of tank milk and in all silo samples. Phenotypic and genetic analysis of 153 isolates identified 151 (98.69%) as Staphylococcus aureus. Amplification of the coa gene was positive for 149 isolates (97.39%). The staphylococcal enterotoxin (SE) C gene was detected in 116 strains (75.82%), whereas more than one SE gene was carried in 5 strains (3.26%). None of the isolates harbored the genes for SEE or for methicillin resistance. A high prevalence of CPS carrying enterotoxin genes should be of concern because ewe milk is mainly processed into raw milk cheeses. The detection of the coa gene from milk samples could help to assess the microbiological safety of raw milk intended for direct use in the dairy industry.
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