Prediction of enteric methane output from milk fatty acid concentrations and rumen fermentation parameters in dairy cows fed sunflower, flax, or canola seeds

Milk fatty acid (FA) composition has been suggested as a means of predicting enteric methane (CH(4)) output in lactating dairy cattle because of the common biochemical pathways among CH(4), acetate, and butyrate in the rumen. Sixteen lactating Holstein cows were used in a Latin square design with four 28-d periods. All diets contained steam-rolled barley, a pelleted supplement, barley silage [45% of dietary dry matter (DM)] and 3.3% added fat (DM basis) from 1 of 4 sources: calcium salts of long-chain FA (palm oil; control) or crushed oilseeds from sunflower, flax, or canola. The objectives of this study were to (1) compare the effect of diets on milk FA profile; (2) model CH(4) production from milk FA composition, intake, and rumen fermentation variables; and (3) test the applicability of CH(4) prediction equations reported in previous studies. Methane (g/d) was measured in chambers (2 animals/chamber) on 3 consecutive days (d 21-23). The test variables included total DM intake (DMI, kg/d; cl 21-23), forage DMI (kg/d; d 21-23), milk yield (kg/d; d 21-23), milk components (d 18-21), milk FA composition (% total FA methyl esters; d 18-21), rumen volatile FA (mol/100 mol; d 19-21), and protozoal counts (d 19-21), and were averaged by chamber and period to determine relationships between CH(4) and the test variables. Milk trans(t)10-, t11-18:1, and cis(c)9t11-18:2 were greater for sunflower seeds compared with the other diets. Forage DMI (correlation coefficient, r = 0.52; n = 32), DMI (r = 0.52; n = 32), and rumen acetate + butyrate:propionate (r = 0.72; n = 16) were positively related to CH(4) (g/d), whereas rumen propionate (r = 0.63; n = 16), milk c9-17:1 (r = 0.64; n = 32), and c11-18:1 (r = 0.64; n = 32) were negatively related to CH(4). The best regression equation (coefficient of determination = 0.90; n = 16) was CH(4) (g/d) = -910.8 (+/- 156.7) x milk c9-17:1 + 331.2 (+/- 88.8) x milk 16:0 iso + 0.0001 (+/- 0.00) x total entodiniomorphs + 242.5 (+/- 39.7). Removing rumen parameters from the model also resulted in a reasonably good estimate (coefficient of determination = 0.83; n = 32) of CH(4). Stepwise regression analysis within diets resulted in greater coefficient of determination and lower standard error values. Predictions of CH(4), using equations from previous studies for the data, set from this study, resulted in a mean overestimation ranging from 19 to 61% across studies. Thus, milk FA alone may not be suitable for developing universal CH(4) prediction equations.