Journal
JOURNAL OF DAIRY RESEARCH
Volume 80, Issue 3, Pages 367-373Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0022029913000320
Keywords
Whey protein; lipid peroxidation; radical scavenging activity; antioxidative peptide; Q-TOF MS
Funding
- State Natural Sciences Foundation of China [31071491]
- National Key Technology R&D Program of China [2012BAD33B05, 2011BAD09B03]
- 863 project [2013AA102207]
- Priority Academic Programme Development of Jiangsu Education Institutions
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To isolate and identify antioxidant peptides from enzymatically hydrolysed whey protein, whey protein isolate was hydrolysed by different protease (trypsin, pepsin, alcalase 2.4L, promatex, flavourzyme, protease N). The hydrolysate generated by alcalase 2.4L had the highest antioxidant activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, superoxide radicals and in a linoleic acid peroxidation system induced by Fe2+. The IC50 values of DPPH and superoxide radical scavenging activities of the hydrolysate decreased significantly (6.89 and 38.88%, respectively) after treatment with macroporous adsorption resin. Seven different peptides showing strong antioxidant activities were isolated from the hydrolysate using consecutive chromatographic methods including gel filtration chromatography and high-performance liquid chromatography. The molecular mass and amino acids sequences of the purified peptides were determined using a Quadrupole time-of-flight mass spectrometer (Q-TOF MS). One of the antioxidative peptides, Trp-Tyr-Ser-Leu, displayed the highest DPPH radical scavenging activity (IC50 = 273.63 mu M) and superoxide radical scavenging activity (IC50 = 558.42 mu M). These results suggest that hydrolysates from whey proteins are good potential source of natural antioxidants.
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