Journal
JOURNAL OF CROHNS & COLITIS
Volume 6, Issue 2, Pages 189-197Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.crohns.2011.08.003
Keywords
Organic cation/carnitine transporters; Crohn's disease; Carnitine
Categories
Funding
- Crohn's and Colitis Foundation of Canada (CCFC)
- Fondation Litta of Geneva, Switzerland
- Canada Research Chair in Immune Mediated GI Disorders
- Bruce Kaufman Endowed Chair in IBD at McGill
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Background: The IBD5 locus is a genetic risk factor for IBD, particularly Crohn's Disease, coding for the organic cation/carnitine transporters (OCTN1 and 2). Two variants of OCTN are associated with susceptibility to Crohn's Disease. Modified transport of carnitine in vitro has been reported for a polymorphism of OCTN1. The aim was to investigate the function of intestinal OCTNs in IBD in relation to genetic polymorphisms. Methods: Intestinal tissue was obtained from endoscopic biopsies and surgical resections from IBD patients (n=33 and 14, resp.) and controls (n=22 and 14, resp.). OCTN protein levels were measured in intestinal biopsies and carnitine transport was quantified in intestinal resections. Results: OCTN1 protein levels were significantly higher in ileal versus colonic tissue (2.95%+/- 0.4 vs 0.66%+/- 0.2, resp.; p<0.0002). OCTN1 expression was higher in Crohn's disease patients with mutant homozygous or heterozygous genotypes (0.6%+/- 0.1 vs 3%+/- 0.8, resp., p<0.02). Carnitine transport was very rapid and Na+ dependent (10 s). It was not different comparing Crohn's Disease and control groups (0.45 +/- 0.12 vs 0.51 +/- 0.12 nM carnitine/mg prot/min, resp.). Carnitine transport tended to be higher in subjects with mutant homozygous and heterozygous OCTN1 and OCTN2 genotypes (0.19 vs 0.59 and 0.25 vs 0.6, respectively). Conclusions: The present data reveal that OCTN protein levels appear to be similar in intestinal tissue from Crohn's Disease patients and controls. Overall, ileal carnitine transport appears to as well equal in Crohn's Disease and control groups. However, there was a trend towards higher carnitine transport in subjects with OCTN1 and OCTN2 mutations. (C) 2011 European Crohn's and Colitis Organisation. Published by Elsevier B.V. All rights reserved.
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