Journal
ACTA BIOMATERIALIA
Volume 20, Issue -, Pages 10-21Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2015.03.041
Keywords
Intervertebral disc; Mesenchymal stem cell; Chondrocyte; Hydrogel; Collagen
Funding
- EU [NMP3-LA-2008-213904]
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Intervertebral disc (IVD) degeneration is one of the main causes of low back pain. Current surgical treatments are complex and generally do not fully restore spine mobility. Development of injectable extracellular matrix-based hydrogels offers an opportunity for minimally invasive treatment of IVD degeneration. Here we analyze a specific formulation of collagen-low molecular weight hyaluronic acid (LMW HA) semi-interpenetrating network (semi-IPN) loaded with gelatin microspheres as a potential material for tissue engineering of the inner part of the IVD, the nucleus pulposus (NP). The material displayed a gel-like behavior, it was easily injectable as demonstrated by suitable tests and did not induce cytotoxicity or inflammation. Importantly, it supported the growth and chondrogenic differentiation potential of mesenchymal stem cells (MSC) and nasal chondrocytes (NC) in vitro and in vivo. These properties of the hydrogel were successfully combined with TGF-133 delivery by gelatin microspheres, which promoted the chondrogenic phenotype. Altogether, collagen-LMW HA loaded with gelatin microspheres represents a good candidate material for NP tissue engineering as it combines important theological, functional and biological features. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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