Correlation between micro-computed tomography and histomorphometry for assessment of new bone formation in a calvarial experimental model

Conventional histologic or histomorphometric evaluation provides clear evidence of the bone healing process. However, the sample preparation process is tedious and destructive, and the three-dimensional (31)) anisotropic information of the bone trabeculae is compromised. Micro-computed tomography ( LCT) has been introduced as an alternative to these traditional evaluation methods. LCT is noninvasive and provides a faster approach to evaluate and quantify cancellous bone. Most previous studies that used LCT have focused on studying trabecular structures of cancellous bone. In this study, we used LCT to analyze the micro-architecture of the regenerated membranous bone using a rabbit cranial defect model. Two 1 cm diameter circular bony defects were created in 12 New Zealand white rabbits. Specimens were harvested at 6 weeks and 12 weeks after surgery and were scanned using a MicroCT machine (Skyscan 1072, Aartselaar, Belgium). The specimens were then sectioned and stained with Goldner's trichrome. Bone volume density (BV/TV), bone surface density (BS/BV), and trabecular thickness (TbTh) were determined from histomorphometric and two-dimensional (213) and 3D mu CT analysis. Pearson's correlation coefficient (gamma), paired t-tests, and intraclass correlation coefficients from measurements between the 2D and 3D LCT and histomorphometry were calculated. There were very strong positive correlations of BV/TV between histomorphometric and 2D or 3D [LCT measurements. Correlation between histomorphometric and 2D mu CT measurements for BS/BV was moderate, whereas correlation between histomorphometric and 3D mu CT measurements was weak. Weak correlations in TbTh among the three methods were found. In conclusion, the present study suggests that, in evaluating micro-architectures in regenerated bones, the correlation between measuring methods vary according to the features measured.