Synthesis, spectroscopic, antibacterial and antifungal studies of nickel(II)5(2'-hydroxyphenyl)-3-(4-X-phenyl)pyrazolinates and their addition complexes with N and P donor ligands

A number of complexes of nickel(II) with 5(2'-hydroxyphenyl)-3-(4-X-phenyl)pyrazolines of the type (C15H12N2O X)2Ni [where X = H, Cl, CH3 and OCH3] were synthesized by the reaction of anhydrous nickel(II) chloride with sodium salts of pyrazoline in 1 : 2 molar ratio. Their addition complexes with 2,2'-bipyridine, 1,10-phenanthroline and triphenylphosphine were prepared in 1 : 1 molar ratio. These complexes were characterized by elemental analyses, molecular weight, magnetic, conductivity, IR, electronic, 1H, 13C, 31P NMR and FAB mass spectral data. All complexes are amorphous. Square planar geometry around nickel confirms the presence of two bidentate pyrazoline ligands in nickel(II)5(2'-hydroxyphenyl)-3-(4-X-phenyl)pyrazolinates. In the addition complexes pyrazoline is monodentate. Bidentate and monodentate pyrazoline was confirmed by IR, 1H, 13C and 31P NMR spectral data. All the metal complexes exhibit very good antibacterial and antifungal activity. Coordination of metal ion has pronounced effect on the microbial activities of the ligand. The brine shrimp bioassay was also carried out to study their in vitro cytotoxic properties; all complexes and adducts display potent cytotoxic activity against Artemia salina.