Journal
JOURNAL OF CONTROLLED RELEASE
Volume 158, Issue 3, Pages 461-469Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2011.12.019
Keywords
Transfection; Cationic lipids; Liposomes; Aminoglycoside; Paromomycin; Neomycin B
Funding
- European Union [018 716]
- Association Francaise contre les Myopathies (Evry, France)
- Vaincre La Mucoviscidose (Paris, France)
- Fondation de France (Paris, France)
- Association Francaise contre les Myopathies
Ask authors/readers for more resources
Cationic lipid-based nonviral gene delivery is an attractive approach for therapeutic gene transfer. Basically, gene transfection can be achieved by using synthetic vectors that compact DNA, forming cationic lipoplexeswhich can interact with the cell plasma membrane by electrostatic interactions. Among the basic components of any cationic lipid, the type of cationic headgroup has been shown to have a major role in transfection efficiency. We have previously reported the DNA transfection potential of vectors characterized by a kanamycin A headgroup. The encouraging transfection results obtained with these compounds prompted us to evaluate the potential of cationic lipids bearing headgroups based on other aminoglycosides. Thus, we herein report the synthesis and gene transfection properties of novel cationic lipids consisting of cholesteryl or dioleyl moieties linked, via various spacers, to paromomycin or neomycin B headgroups. Our results confirm that these new aminoglycoside-based cationic lipids are efficient for gene transfection both in vitro and into the mouse airways in vivo. We also investigated physico-chemical properties of the DNA complexes formed by this particular type of synthetic vectors in order to better understand their structure-activity relationships. (C) 2011 Elsevier B. V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available