Journal
JOURNAL OF CONTROLLED RELEASE
Volume 133, Issue 1, Pages 11-17Publisher
ELSEVIER
DOI: 10.1016/j.jconrel.2008.09.086
Keywords
Paclitaxel; PEGylated nanoparticle; PLGA; Anti-tumoral activity
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The purpose of this study was to develop Cremophor (R) EL-free nanoparticles loaded with Paclitaxel (M), intended to be intravenously administered, able to improve the therapeutic index of the drug and devoid of the adverse effects of Cremophor (R) EL. PTX-loaded PEGylated PLGA-based were prepared by simple emulsion and nanoprecipitation. The incorporation efficiency of PTX was higher with the nano precipitation technique. The release behavior of PTX exhibited a biphasic pattern characterized by an initial burst release followed by a slower and continuous release. The in vitro anti-tumoral activity was assessed using the Human Cervix Carcinoma cells (HeLa) by the MTT test and was compared to the commercial formulation Taxol (R) and to Cremophor (R) EL When exposed to 25 mu g/ml of M, the cell viability was lower for M-loaded nanoparticles than for Taxol (R) (IC50 5.5 vs 15.5 mu g/ml). Flow cytometry studies showed that the cellular uptake of PTX-loaded nanoparticles was concentration and time dependent. Exposure of HeLa cells to Taxol (R) and M-loaded nanoparticles induced the same percentage of apoptotic cells. M-loaded nanoparticles showed greater tumor growth inhibition effect in vivo on TLT tumor, compared with Taxol (R) Therefore, M-loaded nanoparticles may be considered as an effective anticancer drug delivery system for cancer chemotherapy. (C) 2008 Published by Elsevier B.V.
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